{"id":5375,"date":"2019-05-01T11:41:32","date_gmt":"2019-05-01T10:41:32","guid":{"rendered":"http:\/\/www.library.sath.nhs.uk\/research\/?p=5375"},"modified":"2021-03-05T12:30:31","modified_gmt":"2021-03-05T12:30:31","slug":"genetic-deletion-of-the-lipid-raft-protein-caveolin-1-leads-to-worsening-renal-fibrosis-2018","status":"publish","type":"post","link":"https:\/\/www.library.sath.nhs.uk\/research\/2019\/05\/01\/genetic-deletion-of-the-lipid-raft-protein-caveolin-1-leads-to-worsening-renal-fibrosis-2018\/","title":{"rendered":"Genetic Deletion of the Lipid Raft Protein Caveolin-1 Leads to Worsening Renal Fibrosis (2018)"},"content":{"rendered":"

Type of publication:<\/strong>
\nJournal article<\/p>\n

Author(s):<\/strong>
\n*Chand S<\/a> \"\"<\/a>, Hazeldine J, Smith S, Borrows R.<\/p>\n

Citation:<\/strong>
\nJournal of Clinical Nephrology and Renal Care 2018 Jun;4(1):037<\/p>\n

Abstract:<\/strong>
\nBackground
\nRenal disease is a major global public health issue. Renal interstitial fibrosis is the characteristic histopathological finding in all progressive renal disease. Caveolin-1 is the essential structural protein for lipid rafts called caveolae that are ubiquitously distributed among fibroblasts, endothelial and epithelial cells. Caveolin-1 acts as an intracellular signalling pathway chaperone in fibrotic disease. Presently, caveolin-1 expression is associated with more severe renal disease in human and previous murine studies. In non-renal fibrosis, caveolin-1 protects against fibrosis. The purpose of this study was to investigate if caveolin-1 knockout led to an increased fibrotic phenotype using the unilateral ureteric obstruction model of renal fibrosis.
\nMethods
\nUsing 2 time-points of the unilateral ureteric obstruction model, wild-type and caveolin-1 knockout mouse kidneys were analysed for caveolin-1 expression and markers of fibrosis using histology, Gomori staining, real-time quantified polymerase chain reaction, Western blotting and confocal microscopy.
\nResults
\nConfocal microscopy shows caveolin-1 staining mainly in glomerulus, lining of tubules as well as the vasculature. There was increased caveolin-1 expression the longer the unilateral obstruction occurred as well as in the contralateral compensating non-obstructed kidney. Caveolin-1 knockout had less fibrosis at day 3 histologically but more at day 14 as compared to wild-type. There were significantly more F4\/80 positive staining cells at day 3 and day 14 in the wild-type injured kidney as compared to the caveolin-1 knockout mouse.
\nConclusion
\nCaveolin-1 knockout leads to a worse fibrosis upon unilateral ureteric obstruction. Caveolin-1 expression manipulation timing remains to be elucidated in reducing renal fibrosis.<\/p>\n

Link to full-text<\/a>\u00a0[no password required]<\/p>\n","protected":false},"excerpt":{"rendered":"

Type of publication: Journal article Author(s): *Chand S , Hazeldine J, Smith S, Borrows R. Citation: Journal of Clinical Nephrology and Renal Care 2018 Jun;4(1):037 Abstract: Background Renal disease is a major global public health issue. Renal interstitial fibrosis is…<\/span><\/p>\n

Read more Genetic Deletion of the Lipid Raft Protein Caveolin-1 Leads to Worsening Renal Fibrosis (2018)<\/span> ›<\/span><\/a><\/div>\n

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